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Molecular Investigation Department (DIM)


Molecular Investigation Department (DIM) will ensure the approach at the molecular level of new strategic / priority research directions integrated in the overall framework for the complex approach of biological systems and their interactions with the environment within the priority axis research which meet the requirements of the EU Research and Development strategy for environmental and sustainable development.

DIM is structured in 6 modules:

The modules are designed to provide the flexibility necessary for different methodological approaches of various research topics through the most efficient and appropriate equipment currently available in the world.

Genomics

The module is dedicated to the study of genetic information from DNA with the possibility of evaluating the genotypes of normal and modified microorganisms, plants, humans and animals. Genomics serves the following strategic research directions: "Investigation of biodiversity" and "The response of biological systems to the action of environmental / control factors, including climate change". In the module laboratories, we will perform sequencing, genotyping, detection of chromosomal abnormalities, normal and pathological polymorphisms services. The equipment used is the best available and they allow the approach of any research direction / topic and service in the field. The results are analyzed with efficient and specific software programmes, and then they are reported as well as included in international databases (GenomeDataBase).

Transcriptomics

The module ensures the characterization and evaluation of the gene expression profile in a given cell under certain conditions or in response to environmental influences / control factors. Transcriptomics ensures the need for integrated vision and prediction at the molecular level by: i) highlighting the genes expressed specifically under certain conditions (diagnosis, prognosis, therapeutic targets), ii) identifying groups of co-regulated genes (adjustment network, highlighting the potential functions of the product genes), iii) the discovery of new RNA molecules involved in genetic polymorphism, genome structural adnotation  (exon / intron, beginning / end of eukaryotic genes), identification of defective mutants under certain circumstances.

The laboratory module serves the following strategic research directions "The response of biological systems to the action of environmental / control factors, including climate change" and "Biotechnology and renewable resources - Molecular Biotechnology for obtaining bioprocessed products" the specific direction "Obtaining natural compounds with biological protective action in humans and animals" and the achievement of qualitative and quantitative assessment services of gene expression by "RNA microarray" and "Real-Time RT-PCR".

Proteomics

The module pertains to

identifying and studying the entire gene expression at the level of the protein, providing a complete and complex approach of the evaluation in the context of protein-protein, protein-control factor interactions. Proteomics allows the identification of the cellular structure, function and location for new and less abundant proteins.

The laboratories in the module structure combine traditional study techniques and methodology (electrophoresis, chromatography, spectrophotometry, enzyme kinetics, etc.) in the field with new analytical approaches using extremely efficient purification and characterization equipment (FPLC-medium pressure liquid chromatography, HPLC-high pressure liquid chromatography coupled with MS-mass spectrometry, two-dimensional electrophoresis coupled with MS). The module laboratories will be serving directions such as "The response of biological systems to the action of environmental / control factors, including climate change" and "Biotechnology and renewable resources - Molecular Biotechnology for obtaining bioprocessed products" and the more specific direction "Obtaining natural compounds with biological protective action in humans and animals" and the achievement of the qualitative and quantitative assessment services of gene expression at the protein level.

Biochemistry

The new biochemistry laboratories combine the traditional methods of analysis of biochemical parameters (carbohydrates, lipids, proteins / enzymes, nucleic acids, metabolites) with the latest methodology involving a new integrative approach for the assessment of the glycomic lipidomic, metabolomic profile. These new methods have the merit of highlighting the degree of interconnection of various structural and functional components. The Biochemistry Module serves the directions "The response of biological systems to the action of environmental / control factors, including climate change" and "Biotechnology and renewable resources - Molecular Biotechnology for obtaining bioprocessed products" and the more specific direction "Obtaining natural compounds with biological protective action in humans and animals" and the achievement of biochemical parameters, metabolites, enzyme dosage and kinetics, assessment services etc.

Full and complex analysis of the results in order to integrate the findings in the other levels of approach is carried out in collaboration with the Department of Bioinformatics and Modulation (DBIM).

The specialists who will operate within DIM will have the ability and capability to service the research directions related to all current and future research axes / subaxes to be addressed. This includes all activities of strategic and specific research directions submitted and it will involve services to third parties as analyses based on orders as well as through technology transfer activities as follows: i) characterization and evaluation of the effects of biological compounds with protective action in humans and animals; ii) sturgeon genotyping applicable to the characterization of  caviar quality, iii) molecular markers for traceability of meat and meat products;